tlc screening and evaluation of antioxidant, antibacterial activity of onopordon macrocephalum by bioautography method
Authors
abstract
tlc screening and evaluation of antioxidant, antibacterial activity of onopordon macrocephalum by bioautography methodonopordon known as scotch thistle is a medicinal plant of the asteraceae family that is widely distributed in europe and asia, also it has many valuable medicinal properties as hypotensive and antitumor. thin layer chromatography (tlc) is wildly used in natural product extract analysis as a finger print which can be used for identification and quality control of medicinal preparations. performing thin-layer chromatography tlc tests on the plant show the presence of flavonoids, coumarins and bitter principles. planar chromatographic analysis hyphenated with the biological detection method is known as bioautography which is an effective and inexpensive technique for the phytochemical analysis of plant extracts. thin-layer chromatography-direct bioautography links separation on the adsorbent layer with biological tests performed directly on it in order to identify antimicrobial and antioxidant activities. bioautography revealed that staphylococcus aureus was inhibited by most of the flavonoids separated on the tlc plates. similarly, growth of the bacillus cereus was also inhibited by one flavonoid band on tlc. antioxidant bioautography shows the strong antioxidant activity of one flavonoid band, whereas no activity detected of bitter principles. bioautography showed that the antimicrobial and antioxidant activity was probably due to flavonoids.
similar resources
TLC Screening and Evaluation of Antioxidant, Antibacterial Activity of Onopordon Macrocephalum by Bioautography Method
TLC screening and Evaluation of antioxidant, antibacterial activity of Onopordon macrocephalum by bioautography methodOnopordon known as scotch thistle is a medicinal plant of the Asteraceae family that is widely distributed in Europe and Asia, also it has many valuable medicinal properties as hypotensive and antitumor. Thin layer chromatography (TLC) is wildly used in natural product ext...
full textantioxidant and antimicrobial activity of allium jesdianum extract
چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...
Assessment of Bioautography and Spot Screening of TLC of Green Tea (Camellia) Plant Extracts as Antibacterial and Antioxidant Agents
This study was carried out as a prerequisite to evaluate the therapeutic potential of Camellia varieties. The crude extracts of six different plants of green tea Camellia assamica and Camellia sinensis were tested against three Gram-positive and four Gram-negative bacteria using agar disk diffusion method at 50 mg/ml concentration. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and dip...
full textDetection of antibacterial activity of essential oil components by TLC - bioautography using luminescent bacteria
Gy. Horváth: Department of Pharmacognosy, Medical School, University of Pécs, Rókus u. 2., H-7624 Pécs, Hungary B. Kocsis: Institute of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary É. Lemberkovics and A. Böszörményi: Institute of Pharmacognosy, Faculty of Pharmacy, Semmelweis University, Üllői út 26., H-1085 Budapest, Hungary P.G....
full textdetection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
My Resources
Save resource for easier access later
Journal title:
iranian journal of pharmaceutical sciencesجلد ۱۲، شماره ۲، صفحات ۱-۸
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023